Phytochemical Study of Butea superba Roxb. and its Extraction by Successive Solvent and Direct Ethanolic Extraction


  • Kushwaha A. K. School of pharmacy, SunRise University, Alwar, Rajasthan 301028
  • Babar V. B. School of pharmacy, SunRise University, Alwar, Rajasthan 301028



Herbal therapy is a widely accepted therapeutic method, where the use of medicinal herbs has great importance in traditional medicine. Butea superba L., often referred to as Ghandhali, Bakuchi, Khip, and Prasaran, is an indigenous green vegetable originating from India. The herb has been traditionally used to augment physical strength, potency, and male sexual prowess. An essential aspect of research is examining the bioactivity of each component, since it has a substantial impact on the regulation of biological processes and the treatment of numerous conditions such as diabetes, hypertension, asthma, hepatomas, psoriasis, and perhaps cancer. The current investigation centered on the extraction and phytochemical analysis of Butea superba Roxb, using both Successive Solvent Extraction (SSE) and Direct Ethanolic Extraction (DEE) techniques. The SSE technique included a methodical extraction procedure using solvents with progressively higher polarity, while DEE immediately employed ethanol. Phytochemical analysis was performed on the extracted components of Butea superba to determine the presence and amount of bioactive substances. Diverse analytical techniques, such as chromatography, were used to investigate the chemical makeup. This extensive study seeks to provide valuable insights into the efficacy of SSE (Solvent Extraction) and DEE (Deep Eutectic Solvent Extraction) techniques for extracting phytochemicals from Butea superba, as well as to clarify the range of bioactive substances found in this plant.


Download data is not yet available.


Metrics Loading ...


<i>Butea superba</i>, Bioactives, Chromatography, Successive Extraction, Ethanolic Extraction




How to Cite

A. K., K., & V. B., B. (2023). Phytochemical Study of <i>Butea superba</i> Roxb. and its Extraction by Successive Solvent and Direct Ethanolic Extraction. International Journal of Newgen Research in Pharmacy & Healthcare, 1(2), 8–17.





Sirichaiwetchakoon K, Suksuphew S, Srisawat R, Eumkeb G. Butea superba Roxb. Extract ameliorates scopolamine-induced cognitive and memory impairment in aged male rats. Evid Based Complement Alternat Med [Internet]. 2021; 2021:1–15. Available from: DOI:

Binalee F, Udomuksorn W, Kumarnsit E, Vongvatcharanon U, Vongvatcharanon S. Anabolic effect of Butea superba Roxb. On improving skeletal muscle atrophy and strength via androgen receptors and increasing parvalbumin levels in orchidectomized rats. Sci Asia [Internet]. 2022;48(6):718. Available from: DOI:

B S, K K. Qualitative analysis of inorganic acid, basic radicals, and estimation of biomolecules in the flower extract of Butea superba roxb. Asian J Pharm Clin Res [Internet]. 2018;11(10):161. Available from: DOI:

Eumkeb G, Tanphonkrang S, Sirichaiwetchakoon K, Hengpratom T, Naknarong W. The synergy effect of daidzein and genistein isolated from Butea superba Roxb. on the reproductive system of male mice. Nat Prod Res [Internet]. 2017;31(6):672–5. Available from: DOI:

Smitasiri Y, Pinmongkholgul S. Acute effect of Butea superba roxb. Extract on intracavernous pressure and blood pressure in adult male rats. Acta Hortic [Internet]. 2014;(1023):149–53. Available from: DOI:

.Bagchi B, Department of Botany, Bangabasi College, Kolkata-700009, West Bengal, India. A relative study of diversity of endophytic fungi in a Lianas Butea superba from Belpahari and their seasonal variation. International Journal of Experimental Research and Review [Internet]. 2020; 23:27–34. Available from: DOI:

.Malaivijitnond S, Ketsuwan A, Watanabe G, Taya K, Cherdshewasart W. Luteinizing hormone reduction by the male potency herb, Butea superba Roxb. Braz J Med Biol Res [Internet]. 2010;43(9):843–52. Available from: DOI:

Boonsombat J, Prachyawarakorn V, Pansanit A, Mahidol C, Ruchirawat S, Thongnest S. Superbanone, A New 2-Aryl-3-benzofuranone and Other Bioactive Constituents from the Tube Roots of Butea superba. Chem Biodivers [Internet]. 2017;14(7):e1700044. Available from: DOI:

Leelahawong C, Srisomsap C, Cherdshewasart W, Chokchaichamnankit D, Vinayavekhin N, Sangvanich P. Proteomic analysis of isozymes in Butea superba leaves. Russ J Plant Physiol [Internet]. 2017;64(1):100–8. Available from: DOI:

Mizuki D, Qi Z, Tanaka K, Fujiwara H, Ishikawa T, Higuchi Y, et al. Butea superba-induced amelioration of cognitive and emotional deficits in olfactory bulbectomized mice and putative mechanisms underlying its actions. J Pharmacol Sci [Internet]. 2014;124(4):457–67. Available from: DOI:

Mizuki D, Matsumoto K, Tanaka K, Thi Le X, Fujiwara H, Ishikawa T, et al. Antidepressant-like effect of Butea superba in mice exposed to chronic mild stress and its possible mechanism of action. J Ethnopharmacol [Internet]. 2014;156:16–25. Available from: DOI:

Arai MA, Koryudzu K, Koyano T, Kowithayakorn T, Ishibashi M. Naturally occurring Ngn2 promoter activators from Butea superba. Mol Biosyst [Internet]. 2013;9(10):2489–97. Available from: DOI:

Kumar Goswami S, Jamwal R, Balachandran J, Kumar Pandre M, Dethe S, Agarwal A, et al. Multiple enzyme inhibition potential of Butea superba for management of erectile dysfunction. Planta Med [Internet]. 2012;78(11). Available from: DOI:

Tocharus C, Sooksaen P, Shimbhu D, Tocharus J. Butea superba (Roxb.) improves penile erection in diabetic rats: B. superba improves penile erection. Andrologia [Internet]. 2012;44 Suppl 1:728–33. Available from: DOI:

Cherdshewasart W, Sutjit W, Pulcharoen K, Chulasiri M. Mutagenic and antimutagenic effects of the traditional herb used for treating erectile dysfunction, Butea superba Roxb. Biosci Biotechnol Biochem [Internet]. 2010;74(5):923–7. Available from: DOI: